Isobaric tagging methods such as the Thermo Scientific Tandem Mass Tag (TMT) have emerged as the method of choice for relative quantitation of proteins across a high number of samples with good precision and minimal missing values.
Mass resolving power exceeding 50,000 FWHM at low m/z is required to resolve TMT reporter ions and isobaric interferences. Orbitrap instruments are capable of these requirements however at the expense of lower scan speed.
A novel high-resolution accurate mass (HRAM) platform with mass resolving power up to 100,000 FWHM and scan speed up to 200 Hz in MS2 mode was evaluated for its quantitative performance using biological samples of different complexity.